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Global Journal of Physical Chemistry. Volume 2, Issue 2 (2011) pp. 140-144
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Research Article
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Free Article
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Neuritogenesis on antagonist surfaces
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Guillaume Lamoura, Sylvie Souèsa, Ahmed Hamraouia,b
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a UFR Biomédicale, Université Paris Descartes, 45 rue des Saints-Pères, 75270 Paris Cedex 06, France
b Service de Physique et Chimie des Surfaces et Interfaces, CEA Saclay, 91191 Gif-sur-Yvette, France
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Abstract |
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The nanoscale characteristics of the adhesion substrate and the composition of the culture medium are investigated as factors that have an influence on neuritogenesis and on the structure/morphology of the neurites. Poly-L-lysine (PLL) is known to produce a positively charged surface, as opposed to poly-L-ornithine (PLO) which produces a substrate that is very similar in its structure. Lamellipodia are observed all along neurites formed on a PLL-coated glass surface, but not on a PLO-coated glass surface. Collagen is a natural protein that enhances adhesion. Provided the matrix formed by collagen on a glass surface is thin enough, nanoroughness, rather than substrate compliance, is demonstrated to impact cell aggregates and neurite morphology. AFM and optical microscopy are used to illustrate the differences pertaining to PLL, PLO and collagen coated glass surfaces used as culture substrates for neuronal cell culture.
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Keywords |
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Neurite outgrowth; Collagen; Poly-L-lysine; Poly-L-ornithine; Lamellipodia; Nanoroughness
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