A fluorescence derivatization reaction based on the reaction of Nifedipine with o-phtaldialdehyde after a previous reduction of the nitro group to a primary amine and photooxidation of the dihydropyridine to pyridine was optimized. Chemometric data analysis was done by parallel factor analysis 2 (PARAFAC2) multivariate calibration model of a three way data structure obtained by the acquisition of complete excitation-emission matrices (EEMs) of a Nifedipine fluorescent derivate following a standard addition method. The results found in the quantification of Nifedipine in different pharmaceutical formulations were compared with the official US Pharmacopeia high performance liquid chromatography - ultraviolet method (USP HPLC-UV). For each of the pharmaceutical formulations a fortified sample was also analysed. Good results were found with the PARAFAC2 spectrofluorimetric methodology. Generally similar PARAFAC2 models were needed for the adequate decomposition of the unfortified and fortified samples of the same pharmaceutical formulation.