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Global Journal of Analytical Chemistry. Volume 1, Issue 3 (2010) pp. 181-188
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Research Article
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Determination of pteridine levels in biofortified plant samples by capillary electrophoresis with laser induced fluorescence
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Stephen E. Gibbonsa, Tahzeeba Hossainb, Yinfa Maa*
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a Department of Chemistry and Environmental Research Center, Missouri University of Science and Technology, Rolla, MO, 65409, USA
b Donald Danforth Plant Science Center, 975 North Warson Road, Saint Louis, MO, 63132, USA
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Abstract |
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It has been observed through high performance liquid chromatography (HPLC) and thin layer chromatography (TLC) that pteridines are naturally present in plants. Previous research results have shown that folate biofortification of plants through metabolic engineering increases the synthesis of pteridines. Subsequently, the ability to analyze these plant samples and quantify the pteridine levels is one way to determine the effectiveness of the folate biofortification. Although a variety of techniques have been used to measure the pteridine levels in plants, a high resolution separation technique is still needed to overcome the matrix effects in plant tissues. In this paper, we have developed a method to quantitatively determine the levels of nine pteridine compounds in plant samples of wild type non-transgenic and transgenic plants engineered for increased pteridine synthesis using capillary electrophoresis with laser induced fluorescence (CE-LIF).
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Keywords |
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Capillary Electrophoresis; Laser Induced Fluorescence; Pteridine
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