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  Global Journal of Analytical Chemistry. Volume 1, Issue 3 (2010) pp. 181-188
  Research Article
 
Determination of pteridine levels in biofortified plant samples by capillary electrophoresis with laser induced fluorescence
  Stephen E. Gibbonsa, Tahzeeba Hossainb, Yinfa Maa*  
     
a Department of Chemistry and Environmental Research Center, Missouri University of Science and Technology, Rolla, MO, 65409, USA
b Donald Danforth Plant Science Center, 975 North Warson Road, Saint Louis, MO, 63132, USA

   
  Abstract  
  It has been observed through high performance liquid chromatography (HPLC) and thin layer chromatography (TLC) that pteridines are naturally present in plants. Previous research results have shown that folate biofortification of plants through metabolic engineering increases the synthesis of pteridines. Subsequently, the ability to analyze these plant samples and quantify the pteridine levels is one way to determine the effectiveness of the folate biofortification. Although a variety of techniques have been used to measure the pteridine levels in plants, a high resolution separation technique is still needed to overcome the matrix effects in plant tissues. In this paper, we have developed a method to quantitatively determine the levels of nine pteridine compounds in plant samples of wild type non-transgenic and transgenic plants engineered for increased pteridine synthesis using capillary electrophoresis with laser induced fluorescence (CE-LIF).
     
  Keywords  
  Capillary Electrophoresis; Laser Induced Fluorescence; Pteridine  
     
   
   
   
   
     

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