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  Global Journal of Analytical Chemistry. Volume 1, Issue 2 (2010) pp. 146-153
  Research Article
Comparison of methods for the exhaustive extraction of fatty acids of Gastrodia elata Blume
  Yan-Ni Zhangc, Xuan-Feng Yuea,b,c, Jing Zhanga,b, Hong-Lan Qia,b  
a College of Chemistry and Materials Science, Shaanxi Normal University,Xi’an 710062, China
b Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Shaanxi Normal University, Xi’an 710062, China
c Key Laboratory of Ministry of Education for Medicinal Plant Resources and Natural Pharmaceutical Chemistry, Xi’an 710062, China


Supercritical fluid extraction (SFE) was compared with hydrodistillation extraction (HD), Soxhlet extraction (SE) and microwave assistant extraction (MAE) for an analysis of volatile components of Gastrodia elata Blume. The identification and quantitation were conducted on gas chromatography-mass spectrometry (GC-MS). Results showed that SFE turned out to be the preferred among th e four methods in terms of extraction yield and the composition. The effects of variables, including pressure, temperature, extraction time and modifier volume, on SFE yield were investigated. Free fatty acids (FFAs) turned out to be predominant in the volatile fraction by SFE, and the top 5 abundant FFAs were quantified. Palmitic acid was found to be the most abundant (13.0 ± 0.5 mg/g) in the extract by SFE, followed by linoleic acid (10.4 ± 0.4 mg/g) and linolenic acid (2.6 ± 0.1 mg/g).

  Extraction methods; Gastrodia elata Blume; Free Fatty Acids; Supercritical Fluid Extraction  

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