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  Global Journal of Analytical Chemistry. Volume 1, Issue 2 (2010) pp. 130-133
  Research Article
Fluorescence laser-scanning microscopy with one-photon ultrashort pulsed illumination
  Arijit Kumar Dea,b, Debabrata Goswamia*  
a Department of Chemistry, Indian Institute of Technology Kanpur, UP - 208016, India
b Present address: Physical Biosciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA

  The major thrust of modern day fluorescence laser-scanning microscopy has been towards achieving still better depth resolution embodied by the invention and subsequent development of one-photon confocal and multiphoton fluorescence microscopic techniques. However, each method bears its own limitations in having sufficient background fluorescence resulting from out-of-focus illumination for the former while low multiphoton absorption cross-sections of common fluorophores for the latter. Based on our recent communication published elsewhere [De, A. K. and Goswami, D., “Three-dimensional image formation under one-photon ultra-short pulsed illumination”, Proc. SPIE, 7378 (2009), 737827 (1-4)], here we describe in detail how one-photon ultrafast pulsed excitation leads to a consortium between confocal and multiphoton fluorescence microscopy.
  Fluorescence microscopy; Optical sectioning; Confocal detection; Multiphoton excitation; pulsed illumination; Ultra-short pulses; Second-harmonic generation  

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