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  Global Journal of Analytical Chemistry. Volume 2, Issue 5 (2011) pp. 214-223
  Research Article
Simultaneous determination of mangiferin, ferulic acid, berberine, palmatine and icariin in rat plasma after oral administration of Erxian Decoction for their pharmacokinetic studies by LC-ESI-MS analysis
  Yongmei Hua,*, Dongmei Wangb, K. W Tsanga, Qing Liua, Lidan Zhonga, Yao Tonga,*  
a School of Chinese Medicine, the University of Hong Kong, 10 Sassoon Road, Pokfulam, Hong Kong, China
b Department of Pharmaceutics, Sun-Yet University, Guangzhou, 510006, P. R. China

  A sensitive and reliable LC-ESI-MS/MS method was developed for the simultaneous quantification of five representative compounds of mangiferin, ferulic acid, berberine, palmatine, and icariin, in rat plasma. Plasma samples were pretreated by protein precipitation method using acetonitrile-methanol (1:1, v/v). Chromatographic separation was performed on an XBridge C18 column (2.1 mm × 150 mm, 5.0 µm, WAT 003110) using a gradient solvent system of water (containing 0.1% formic acid and 2mM ammonium acetate) and acetonitrile as mobile phase. The ESI-MS spectra were obtained both in positive and negative selective ion monitoring (SIM) mode. The method was linear over the concentration ranges of 0.5-1300 ng/mL for the tested analytes. The intra- and inter-day precision (RSD) values were less than 12% and the recoveries of all the analytes were between 68~99.1% with the RSD values being less than 14%, respectively. The validated LC-ESI-MS/MS method could be successfully applied to the primary pharmacokinetic study of the five selected markers after oral administration of the effective Erxian Decoction (EXD) fraction.
  LC-ESI-MS/MS; Erxian Decoction (EXD); Pharmacokinetics; Simultaneous quantification  

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