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  Global Journal of Analytical Chemistry. Volume 2, Issue 1 (2011) pp. 46-49
  Research Article
 
A new chromogenic spray reagent for detection of hydrogen cyanamide
  Krishna V. Kulkarnia,*, Davanand B. Shindeb, Dhananjay V. Manec, Raghunath B. Toched,*, Manchk V.Garada  
     
a Directorate of Forensic Science Laboratory, Vidyanagari, Kalina, Santacruz (E), Mumbai, India
b Department of Chemical Technology, Dr Babasaheb Ambedkar Marathwada University, Aurangabad, India
c Department of Chemistry, Chatrapati Shivaji College, Omerga, India
d Department of Chemistry, K.T.H.M. College, Nashik, India

   
  Abstract  
  A new inexpensive chromogenic spray reagent for HPTLC was developed for the detection of hydrogen cyanamide in biological materials. Hydrogen cyanamide in alkaline medium followed by the spray of sodium nitroprusside and potassium ferricyanide gives pink color complex. Such pink color complex is not observed with other insecticides such as carbamate, organophosphorous, organochloro, and pyrethroid insecticides and even the constituents of viscera that consist of amino acids, proteins. The detection limit of hydrogen cyanamide is ca. 0.5 ?g.
     
  Keywords  
  Chromogenic reagent; Forensic science; HPTLC; Spray reagent; Hydrogen cyanamide  
     
   
   
   
   
     

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