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Global Journal of Analytical chemistry. Volume 2, Issue 6 (2011) pp. 241-271
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Review Article
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Free Article
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There´s plenty of light at the bottom:
Bioanalytical and biomedical applications of luciferases
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Simone M. Marques, Joaquim C.G. Esteves da Silva
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Centro de Investigação em Química da Universidade do Porto (CIQ-UP), Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Rua do Campo Alegre, no. 687, 4169-007 Porto, Portugal
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Abstract |
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Bioanalytical chemistry has been attracting much attention in recent years due to the growing interest in biomolecules in the biochemical, biological, biomedical, pharmaceutical and environmental contexts, allowing detailed and sensitive analysis from the molecular up to the whole-body level. This review focuses on the application of luciferases, a generic name for enzymes whose biochemical reactions lead to a particular phenomenon called bioluminescence, concerning bioanalytical chemistry and biomedicine. Several powerful techniques employing luciferases were developed, among which bioluminescence resonance energy transfer (BRET) assays, split luciferase assays and bioluminescence imaging (BLI) stand out. Luciferase bioluminescent methods were successfully applied to gain insights into drug screening, gene-mediated therapy evaluation, infection progression, cancer therapy, cell tracking, biosensors, basic research and biomedical engineering, which will be illustrated through representative examples.
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Keywords |
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Luciferase; Bioluminescence; Bioanalytical chemistry; Biomedicine; Bioluminescence imaging; Bioluminescence resonance energy transfer; Split luciferase; Quantum dot
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