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  Global Journal of Analytical Chemistry 2012, 3: 3
  Research Article
 
Alternative LC–MS/MS method for simultaneous determination of artemether and dihydroartemisinin in human plasma and its application to a bioequivalence study
  Satish G. Pingale, Ketan K. Nerurkar, Sudhir S. Pawar, Kiran V. Mangaonkar  
     
Analytical Chemistry Research Laboratory, Mithibai College Vile Parle (W), 400056, Mumbai, India
   
  Abstract  
  An alternative rapid and sensitive liquid chromatography–tandem mass spectrometry method has been developed and validated for simultaneous analysis of Artemether (ART) and Dihydroartemisinin (DIH) in human plasma. The analytes were extracted from human plasma by liquid-liquid extraction. Artemisinin (ARN) was used as an internal standard for Artemether and Dihydroartemisinin. A Betasil C18 column provided chromatographic separation of analytes followed by detection with mass spectrometry. The method involves simple isocratic chromatography conditions and mass spectrometric detection in the positive ionization mode using an API-3000 system. The proposed method has been validated with linear range of 4–250 ng/mL for both analytes. The inter-run and intra-run precision values are within 5.27, 3.34% for ART and 5.65, 4.18% for DIH at LOQ levels. The overall recoveries for ART and DIH were 74.13 and 67.06%, respectively. Total elution time was 5.00 min. This validated method was used successfully for analysis of plasma samples from a bioequivalence study.
     
  Keywords  
  Column liquid chromatography; Tandem mass spectrometry; Artemether; Dihydroartemisinin; Artemisinin; Human plasma  
     
   
   
   
   
     

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