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Global Journal of Analytical Chemistry. Volume 2, Issue 6 (2011) pp. 285-291
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Research Article
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ELISA of dichlorprop by digital image analysis
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F. G. Sáncheza, A. Navas Díaza, B. L. Pabloa, M. Algarrab, A. Aguilara, E. N. Baroc
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a Department of Analytical Chemistry, Faculty of Sciences, University of Málaga,
Campus de Teatinos s/n. 29071 Málaga, Spain
b Centro de Geologia do Porto, Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal
c Departamento de Ingeniería de Comunicaciones, ETS de Ingeniería de Telecomunicaciones, University of Málaga, Campus de Teatinos s/n. 29071 Málaga, Spain
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Abstract |
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An image based detection of enhanced chemiluminescence enzyme-linked immunosorbent assay (CL-ELISA) has been developed for the quantification of dichlorprop with human γ-Globuline (HGG). Data acquisition on microtiter wells is performed using an economical charge coupled device (CCD) camera for capturing images. The standard curve was produced for 0.1-5000 mg/L of dichlorprop. The minimum detectable concentration was 4.5 mg/L and the relative standard deviation was 0.22% (n=10) for a 10 mg/L sample concentration.
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Keywords |
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Chemiluminescence; Dichlorprop ; ELISA; Digital Image
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