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Global Journal of Analytical Chemistry 2012, 3: 12
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Research Article
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Comparison of gas chromatography-isotope dilution mass spectrometry with chemical derivatization and liquid chromatography-isotope dilution mass spectrometry for the determination of glucose in human serum
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Can Quan, Yuxing Shen, Zhaoting Ding
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National Institute of Metrology, Beisanhuan Donglu No.18, Chaoyang District, 100013, Beijing, China
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Abstract |
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Two sensitive, accurate and precise methods including gas chromatography-isotope dilution mass spectrometry (GC/ID-MS) with chemical derivatization and liquid chromatography-isotope dilution mass spectrometry (LC/ID-MS) with protein precipitation have been developed for the determination of glucose concentration in human serum using 13C6-labeled glucose as internal standard. The two different methods gave the identical results, and the relative standard deviations of GC/ID-MS and LC/ID-MS were respectively 1.0% and 0.47%. The GC/ID-MS method was validated by NIST SRM965a and applied to CCQM K11.1 samples determination organized by CCQM (Consultative Committee for Amount of Substance). And the LC/ID-MS method was validated by linearity of the method, r2=0.9995, with recoveries of spiked samples in the range 100.1% to 102.8%. Both the methods can be used as candidate reference method for evaluation of different routine methods for determination of glucose in human serum.
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Keywords |
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Gas chromatography-mass spectrometry; Liquid chromatography-mass spectrometry; Chemical derivatization; Isotope dilution assay; Glucose; Human serum
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