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  Global Journal of Analytical Chemistry 2012, 3: 12
  Research Article
 
Comparison of gas chromatography-isotope dilution mass spectrometry with chemical derivatization and liquid chromatography-isotope dilution mass spectrometry for the determination of glucose in human serum
  Can Quan, Yuxing Shen, Zhaoting Ding  
     
National Institute of Metrology, Beisanhuan Donglu No.18, Chaoyang District, 100013, Beijing, China
   
  Abstract  
  Two sensitive, accurate and precise methods including gas chromatography-isotope dilution mass spectrometry (GC/ID-MS) with chemical derivatization and liquid chromatography-isotope dilution mass spectrometry (LC/ID-MS) with protein precipitation have been developed for the determination of glucose concentration in human serum using 13C6-labeled glucose as internal standard. The two different methods gave the identical results, and the relative standard deviations of GC/ID-MS and LC/ID-MS were respectively 1.0% and 0.47%. The GC/ID-MS method was validated by NIST SRM965a and applied to CCQM K11.1 samples determination organized by CCQM (Consultative Committee for Amount of Substance). And the LC/ID-MS method was validated by linearity of the method, r2=0.9995, with recoveries of spiked samples in the range 100.1% to 102.8%. Both the methods can be used as candidate reference method for evaluation of different routine methods for determination of glucose in human serum.
     
  Keywords  
  Gas chromatography-mass spectrometry; Liquid chromatography-mass spectrometry; Chemical derivatization; Isotope dilution assay; Glucose; Human serum  
     
   
   
   
   
     

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