Hypoxen is clinically effective in surgical treatment
of benign prostatic hyperplasia and a number of other pathologies. However,
molecular mechanisms underlying the effects of hypoxen have not been clearly identified.
This study was designed to examine if hypoxen affects Na+,K+-ATPase, glycolytic and
antioxidant enzyme activities in human erythrocytes. Cells were treated with
different concentrations of hypoxen and then enzyme activities were
determinated spectrophotometrically.
Hypoxen at 56 µM did
not change Na+,K+-ATPase, hexokinase,
phosphofructokinase, pyruvate kinase, and lactate dehydrogenase activities and at
1.4–14 µM, activities of superoxide dismutase,
catalase, glutathione peroxidase and glutathione reductase. 28 and 56 µM
hypoxen increased superoxide dismutase activity by 66% and 94% (p < 0.001),
decreased glutathione peroxidase activity by 17% and 21% (p < 0.02),
respectively, and had no effect on catalase and glutathione reductase
activities. These effects of hypoxen can contribute to post-operative wound
healing at a faster rate, to postoperative improvements in clinical and
laboratory features, and to a decrease in the risk of postoperative
complications.