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Global Journal of Biochemistry. Volume 2, Issue 4 (2011) pp. 215-249
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Research Article
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Free Article
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Mesendoderm gene regulatory networks in zebrafish
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Chung-Hao Chaoa,b,e, Wen-Fang Tsenga,e, Tzu-Min Chana, Hua-Ling Chena, Chang-Ben Huanga, Te-Hsuan Janga, Hsu Hong-Yia, Horng-Dar Wangb, Chiou-Hwa Yuha,c,d
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a Division of Molecular and Genomic Medicine, National Health Research Institutes,
35 Keyan Road, Zhunan Town, Miaoli County 350, Taiwan, R. O. C
b College of Life Science and Institute of Biotechnology, National
Tsing-Hua University,Hsin-Chu, Taiwan, R. O. C
c College of Life Science and Institute of Bioinformatics and Structural Biology,
National Tsing-Hua University, HsinChu, Taiwan, R. O. C
d Department of the Biological Science & Technology, National Chiao
Tung University, Hsin-Chu, Taiwan, R. O. C
e R2-3033, #35 Keyan Road, Zhunan Town,
Miaoli County 350, Taiwan, R.O.C
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Abstract |
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The genomic program for development operates mainly by the regulated expression of genes encoding transcription factors and the signaling pathways. Complex genetic regulatory networks control developmental fate. In this study we built the mesendoderm GRNs in zebrafish by integration of the spatial and temporal expression pattern with the interaction relationship from literatures searching and perturbation analysis. We established the subcircuits between specific transcription factors using morpholinos against them, and measured certain gene expression profiles by real time quantitative RT-PCR (Q-PCR) and validation by in situ hybridization. From our experiments, we confirmed some interactions were identified from literature, and we also identified new target genes downstream of those candidates, which were not found in the literatures search. Some of the interaction nodes were identified through computational searching of the conserved non-coding region, and validated by functional analysis. This is the systematic approach toward transcriptional regulation in zebrafish development, it give new insight to evolution of deuterostome.
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Keywords |
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Transcription regulation; Gene regulatory network; Zebrafish endoderm development; In situ hybridization
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