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Global Journal of Biochemistry. Volume 2, Issue 2 (2011) pp. 124-133
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Research Article
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Free Article
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Modeling and optimization of lipase-catalyzed hydrolysis of salmon oil for facile and efficient concentration of omega 3 PUFA
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Derya Kahveci, Xuebing Xu
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Department of Molecular Biology, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark
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Abstract |
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Farmed salmon oil with low content of omega 3 polyunsaturated fatty acids (PUFA) was hydrolyzed by Candida rugosa lipase in order to concentrate omega 3 polyunsaturated fatty acids. Response surface methodology (RSM) was applied to optimize the reaction conditions. Temperature, time, enzyme load, and water-to-oil ratio were the factors investigated. Total omega 3 PUFA content, as well as eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA) ratio in the product and oleic acid (OA)/total omega 3 PUFA in released free fatty acids (FFA) were the responses of interest. Maximizing all three responses was aimed, in order to have a high omega 3 PUFA content by a selective hydrolysis. Results showed that all three of the second-order polynomial models adequately fitted the experimental data. Optimum conditions were selected as follows: 45oC of temperature, 4 h of time, 3% enzyme (wt% based on oil amount), 3.16:1 water-to-oil (v/w). Total omega 3 PUFA content of the product at the optimum conditions was 33.01% (wt%), while EPA/DHA was equal to 0.48 and OA/total omega 3 in the FFA fraction was equal to 9.53. The models generated for the responses were proved to be predictive when reaction was scaled up by ~67-fold.
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Keywords |
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Candida rugosa lipase; Hydrolysis; Omega 3 PUFA; Salmon oil; Response surface methodology
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