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  Global Journal of Biochemistry. Volume 2, Issue 2 (2011) pp. 134-141
  Research Article Free Article
 
Evaluation and regulation of the lignocellulolytic activity of novel white-rot basidiomycetes
  Vladimir Elisashvilia, Tamas Torokb, Eva Kachlishvilia, Tamar Khardziania, Eka Metrevelia, Aza Kobakhidzea, Iveta Berikashvilia  
     
a Durmishidze Institute of Biochemistry and Biotechnology (DIBB), 10 km Agmasheneblis kheivani, 0159 Tbilisi, Georgi
b Lawrence Berkeley National Laboratory, 1 Cyclotron Rd., Berkeley, CA 94720, USA

   
  Abstract  
  White-rot basidiomycetes (WRB) varied significantly in their ability to produce lignocellulolytic enzymes. In submerged fermentation on mandarin peels, endoglucanase and xylanase activities of tested fungi varied from 1.2 to 23.3 U/ml and from 3.0 to 60.2 U/ml, respectively. Cerrena unicolor and Coriolopsis gallica proved to be outstanding producers of laccase and displayed enzyme activities of 90-102 U/ml. In addition, C. unicolor produced extremely high manganese peroxidase activity (MnP). C. unicolor was capable to produce high levels of laccase in the presence of mono-, di-, and polysaccharides, and glycerol, whereas Ganoderma lucidum, Pleurotus ostreatus, and Pseudotrametes gibbosa secreted essential laccase activities in the presence of cellobiose, xylan, and lactose, respectively. Supplementing the medium with ammonium sulfate in submerged fermentation on banana peels caused Cerrena maxima to increase laccase and MnP yields 4- and 5-fold as compared to the control. Cu2+ positively stimulated laccase and MnP secretion by C. maxima: 4-fold and 3-fold increase in enzyme activity, respectively, was observed compared to the control. It is evident that one of WRBs’ specific features is their extremely wide biodiversity. They lack a set of universal conditions equally appropriate for their successful cultivation and ligninolytic enzymes production.
     
  Keywords  
  Basidiomycetes; Cellulase; Xylanase; Laccase; Manganese peroxidase; Synthesis  
     
   
   
   
   
     

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