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Global Journal of Biochemistry 2012, 3: 6
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Review Article
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Free Article
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Bioconjugation reactions for covalent coupling of proteins to gold surfaces
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Yih Horng Tan, Binod Pandey, Abeera Sharma, Jay Bhattarai, Keith J. Stine
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Department of Chemistry and Biochemistry and Center for Nanoscience, University of Missouri – Saint Louis Saint Louis, MO 63121
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Abstract |
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Immobilization of proteins on surfaces has numerous potential applications in fields such as biosensor development, microarray technology, drug candidate screening, affinity chromatography, and enzyme technology, as well as in understanding the basic relationship between protein structure and ligand binding. Important technological innovations have been made; however, considerable development is still required to optimize methods for immobilizing proteins. Efforts aimed at the optimization of different immobilization chemistries in order to achieve good surface coverage have been vigorously pursued. The aim of this review is to highlight some creative and widely used protein immobilization strategies, such as the use of self-assembled monolayers (SAMs) as platforms for studying biomolecular recognition and assembly of proteins on surfaces via physical, covalent, and bioaffinity immobilization approaches. A number of well-established analytical techniques for characterizing modified surfaces will be briefly mentioned in the context of the examination of the use of functionalized SAMs for protein immobilization.
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Keywords |
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Protein immobilization; Self-assembled monolayers; Nanobiotechnology; Bioconjugation: Nanoporous gold
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