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Global Journal of Biochemistry 2012, 3: 10
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Research Article
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Grape seed proanthocyanidin extract attenuates DNA fragmentation and oxidant damage in various tissues of rats with hyperthyroidism
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Dina Abdel-Salam Elazazya, Sherine Maher Rizkb, Maged Abdel-Hakeem Barakatb, Rokaya Mahmoud Husseina
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a Hormone Research Centre, National Organization for Drug Control and Research, Cairo, Egypt
b Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt
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Abstract |
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The present study was designed to investigate the protective effects of grape seed proanthocyanidin extract (GSPE) on oxidative damage in rats with experimentally induced hyperthyroidism. Hyperthyroidism was induced by subcutaneous injection of 500 µg/kg/day L-thyroxine for 2 weeks. The experimental animals were divided into five groups: negative control, hyperthyroidism, hyperthyroidism + GSPE in a dose of 25 mg/ kg/day for 2 weeks, hyperthyroidism + GSPE in a dose of 50 mg/kg/day for 2 weeks, and hyperthyroidism + vitamin C in a dose of 100 mg/kg/day for 2 weeks. Hyperthyroidism induced elevation in serum concentrations of triiodthyronine (T3) and thyroxine (T4) and decrease in thyroxine-stimulating hormone (TSH) levels did not significantly change in response to GSPE. DNA fragmentation, thiobarbituric acid reactive substances (TBARS) and nitric oxide (NO) levels were higher in brain, liver and kidney of rats of the hyperthyroid group compared to controls. Glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) levels decreased significantly in the hyperthyroid group compared to control group. Treatment of hyperthyroid rats with GSPE decreased the elevated TBARS and NO levels and DNA fragmentation and increased the lowered GSH, SOD and CAT levels to control levels. In conclusion, our results indicate that GSPE is beneficial as a protective agent against oxidative stress induced by hyperthyroidism in rats.
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Keywords |
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Hyperthyroidism; Grape seed proanthocyanidin extract; DNA fragmentation; Oxidative stress
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