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Global Journal of Biochemistry 2012, 3: 11
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Research Article
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Immunohistological analysis of six new primary pancreatic adenocarcinoma cell lines
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Felix Rückerta, Kristin Wernera, Daniela Austb, Sandra Heringc, Hans-Detlev Saegera, Robert Grützmanna, Christian Pilarskya
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a Department of Visceral, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus, Technische Universität Dresden, Germany
b Institute of Pathology, University Hospital Carl Gustav Carus, Technische Universität Dresden, Germany
c Institute of Legal Medicine, University Hospital Carl Gustav Carus, Technische Universität Dresden, Germany
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Abstract |
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Introduction: Pancreatic ductal adenocarcinoma is an aggressive tumor; treatment remains a challenge because of the lack of effective therapeutic strategies. Basic research in this field is dependent on the availability of model systems. New pancreatic cancer cell lines are therefore important for the study of its biology. In the present study, we report the establishment and characterization of six new pancreatic cancer cell lines (PaCaDD-141, -159, -161, -165, -183, -188). Experimental: All cell lines were derived from pancreatic ductal adenocarcinomas by the Dresden outgrowth protocol. The six cell lines originated from different sample locations. We characterized the cell lines by examining their morphology and their cytostructural and functional profiles. Results and Discussion: All cell lines were cultured in optimized Dresden-Medium. The doubling time ranged from 20 to 43 hours. KRAS mutations were detected in four of the six cell lines. Immunohistochemical staining showed cytoplasmic expression of CK8/18, mostly membrane and partially cytoplasmic expression of E-cadherin and strong expression of ezrin in all cell lines. Three cell lines showed nuclear p53 accumulation and heterogeneous expression of vimentin. SMAD4 was heterogeneously expressed in the cell lines. Conclusions: We were able to establish six new primary pancreatic carcinoma cell lines. As applicable tools for basic research, these cell lines might contribute to a better understanding and treatment of this aggressive tumor.
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Keywords |
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Cell lines; Pancreatic cancer; Outgrowth method
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