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Chemical Sensors 2012, 2: 4
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Research Article
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Poly(xylenol orange) film modified carbon paste electrode as an electrochemical sensor for the determination of dopamine in the presence of ascorbic acid and uric acid: A voltammetric study
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S. Sharath Shankar, Bahaddurghatta E. Kumara Swamy, Bannanakere N. Chandrashekar, Kudur J. Gururaj
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Department of P.G. Studies and Research in Industrial Chemistry, Kuvempu University,
Jnana Sahyadri, Shankaraghatta, Shimoga 577 451, Karnataka, India
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Abstract |
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The carbon paste electrode was modified with xylenol orange by electropolymerization and it was used for simultaneous determination of dopamine (DA), ascorbic acid (AA) and uric acid (UA) in 0.2 M phosphate buffer of pH 7.4. Based on its strong electrocatalytic action towards the oxidation of dopamine, uric acid and ascorbic acid, the overlapping voltammetric response of these biomolecules at the bare electrode is resolved into three well defined voltammetric peaks with lowered oxidation potential and significant increase in the anodic peak current. The poly(xylenol orange) modified carbon paste electrode used for the detection of dopamine was stable and reproducible. The effect of scan rate, pH and concentration was studied. The effect of interferences was studied by differential pulse voltammetric technique. The modified electrode was used for the analysis of DA and UA in real samples with satisfactory results.
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Keywords |
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Carbon paste electrode; Xylenol orange; Dopamine; Biosensor
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