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Chemical Sensors 2012, 2: 13
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Research Article
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Detection of silver ions using hairpin DNA probes and thiazole orange
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Hui Xua, Pingping Xua, Quanwen Liua, Shengxiao Zhanga, Lihua Wangb, Chunhai Fanb
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a School of Chemistry and Material Sciences, Ludong University, Yantai 264025, China
b Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
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Abstract |
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Thiazole orange (TO) is an asymmetric cyanine. It has negligible fluorescence in solution, and obtains intense fluorescence when bound to nucleic acids. A silver specific DNA (SSD) forms a hairpin structure from a random coil structure in the presence of silver ion (Ag+). TO interacts with SSD and SSD/Ag+ in different ways and TO can be used as a fluorescent probe for ssDNA/hairpin structure recognition. Since the Ag+ specifically binds to the SSD, the SSD/TO assembly can also be used as a platform to sense the Ag+ with high selectivity and sensitivity. 3.6 nM is the calculated detection limit from experimental results. The method demonstrated here does not need labeling and is simple and convenient.
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Keywords |
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Silver specific DNA; Thiazole orange; Silver ions
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