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  Chemical Sensors 2012, 2: 13
  Research Article
 
Detection of silver ions using hairpin DNA probes and thiazole orange
  Hui Xua, Pingping Xua, Quanwen Liua, Shengxiao Zhanga, Lihua Wangb, Chunhai Fanb  
     
a School of Chemistry and Material Sciences, Ludong University, Yantai 264025, China
b Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China

   
  Abstract  
  Thiazole orange (TO) is an asymmetric cyanine. It has negligible fluorescence in solution, and obtains intense fluorescence when bound to nucleic acids. A silver specific DNA (SSD) forms a hairpin structure from a random coil structure in the presence of silver ion (Ag+). TO interacts with SSD and SSD/Ag+ in different ways and TO can be used as a fluorescent probe for ssDNA/hairpin structure recognition. Since the Ag+ specifically binds to the SSD, the SSD/TO assembly can also be used as a platform to sense the Ag+ with high selectivity and sensitivity. 3.6 nM is the calculated detection limit from experimental results. The method demonstrated here does not need labeling and is simple and convenient.
     
  Keywords  
  Silver specific DNA; Thiazole orange; Silver ions  
     
   
   
   
   
     

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