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  Chemical Sensors 2013, 3: 3
  Research Article
 
A novel bio-interphase composed of layer-by-layer (graphene oxide-chitosan/cytochrome c-horseradish peroxidase)n multilayers and its electrocatalytic redution of oxygen
  Yu Wangb, Yonghai Songa,b, Hongyu Liub, Lingli Wanb, Li Wanga,b  
     
a Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang 330022, People’s Republic of China
b College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, People’s Republic of China

   
  Abstract  
  A novel bio-interphase composed of graphene oxide and chitosan nanocomposites, cytochrome c and horseradish peroxidase was developed by layer-by-layer assembling graphene oxide-chitosan and cytochrome c-horseradish peroxidase on a gold electrode surface. The assembly process was monitored by atomic force microscopy, UV-vis spectroscopy and electrochemical technique. The (graphene oxide-chitosan and cytochrome c-horseradish peroxidase)n multilayer showed a nearly linear increase in the amount of electroactive proteins up to 5 bilayers, suggesting that electrons are transferred over long distance within the multilayers. The (graphene oxide-chitosan and cytochrome c-horseradish peroxidase)n multilayer exhibited good electrocatalytic response to reduction of oxygen, indicating a multi-step electron transfer chain from electrode via the cytochrome c and horseradish peroxidase to oxygen. The electrocatalytic reduction of oxygen was investigated in detail.
     
  Keywords  
  Layer-by-layer; Graphene oxide; Chitosan; Cytochrome c; Horseradish peroxidase; Oxygen  
     
   
   
   
   
     

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