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Chemical Sensors 2013, 3: 4
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Research Article
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Electrochemical detection of bovine serum albumin via conducting polymer deposited electrode
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Filiz Kuralaya, Gulcin Bolatb, Hande Torunb, Serdar Abacib
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a Department of Chemistry, Faculty of Science and Arts, Ordu University, 52200 Ordu, Turkey
b Department of Chemistry, Faculty of Science, Hacettepe University, 06800 Beytepe-Ankara, Turkey
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Abstract |
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This work details electrochemical detection of bovine serum albumin (BSA) using conducting polymer coated platinum (Pt) electrode. Conducting polymer, poly(vinylferrocenium) (PVF+) exhibits good, stable and reversible electrochemistry at low potentials. Electroactivity of the conducting polymer diminished after BSA immobilization in the study. Change in the electrochemical behavior of the conducting polymer coated electrode was used as an indicator for BSA detection. The effects of polymeric film thickness, immobilization time of BSA and BSA concentration were examined in order to obtain optimum experimental conditions. Characterization methods, scanning electron microscopy (SEM) and atomic force microscopy (AFM) were used to characterize the polymer coated and BSA immobilized polymer coated electrodes. The detection limit was found to be 0.13 mg mL-1 for BSA.
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Keywords |
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Conducting polymer; Redox polymer; Electrochemical detection; Protein; BSA
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