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Chemical Sensors 2014, 4: 9
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Research Article
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Study of fluorescence of doxorubicin in muscle tissue using highly sensitive fluorescence sensing
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Iva Blazkovaa, Marketa Vaculovicovaa,b, Tomas Eckschlagerc, Marie Stiborovad, Libuse Trnkovaa,b,e, Vojtech Adama,b, Rene Kizeka,b
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a Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic, European Union
b Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic, European Union
c Department of Paediatric Haematology and Oncology, 2nd Medical School, Charles University and University Hospital Motol, V Uvalu 84, CZ-150 06 Prague 5, Czech Republic, European Union
d Department of Biochemistry, Faculty of Science, Charles University in Prague, Albertov 2030, CZ-128 40 Prague 2, Czech Republic, European Union
e Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic, European Union
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Abstract |
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Monitoring the behaviour of biologically active compounds in the body is the key to understand their effect. Optical methods are relatively cheap non-ionizing techniques based on the specific optical properties and are an important tool for non-invasive and objective diagnosis with still better and better resolution. In this work, a characterization of fluorescence behaviour of doxorubicin in different environments (ethanol, methanol, dimethyl sulphoxide) has been established. In addition, its spatial distribution in muscle tissue was monitored using fluorescence imaging. Autofluorescence of the tissue was removed by software to limit the effect of the matrix. The novelty of this study lies in the field of sensing of doxorubicin in a tissue. The results can be base for an optic array, which could be used for sensing if the drug in vivo.
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Keywords |
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Doxorubicin; Fluorescence; Nanomedicine; Fluorescence spectroscopy; In vivo imaging; Nanotechnology
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