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Chemical Sensors 2014, 4: 7
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Research Article
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Amperometric disposable magnetoimmunosensor for the determination of fibrinogen in plasma
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Rebeca M. Torrente-Rodríguez, S. Campuzano, M. Gamella, F. Conzuelo, A. J. Reviejo, J. M. Pingarrón
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Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Avda. Complutense s/n, 28040 Madrid, Spain
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Abstract |
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An amperometric disposable immunosensor for fibrinogen (Fib) detection involving magnetic beads (MBs) and screen-printed carbon electrodes (SPCEs) is described for the first time. An indirect competitive configuration was employed relying on the competition between target Fib in solution and biotinylated Fib (Biotin-Fib) immobilized on Streptavidin-modified MBs (Strep-MBs) for binding to a fixed amount of HRP-labelled detection antibody. The MBs bearing the immunoconjugates were magnetically captured on the surface of SPCEs and the catalytic currents measured at -0.20 V (vs. Ag pseudo-reference electrode) upon addition of H2O2 and using hydroquinone (HQ) as redox mediator in solution, were used to monitor the affinity reaction. The methodology allowed an excellent analytical performance to be obtained with a dynamic range (DR) between 0.004 and 0.8 µg mL-1 and a detection limit (LOD) of 0.8 ng mL–1. The developed magnetoimmunosensor was successfully applied to the analysis of an international standard for Fib plasma, after just a dilution in the buffer assay as sample treatment, in a total assay time of 80 minutes.
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Keywords |
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Fibrinogen; Electrochemical immunosensor; Competitive format; Magnetic beads; Plasma
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