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  Chemical Sensors 2014, 4: 6
  Research Article
Facilely prepared nanocomposite of polyaniline-polydopamine-glucose oxidase with enhanced sensing properties for amperometric detection of glucose
  Yang Li, Qianqian Lin, Mujie Yang  
MOE Key Laboratory of Macromolecule Synthesis and Functionalization, Department of Polymer Science and Engineering, Cyrus Tang Center for sensor Materials and Applications, Zhejiang University, Hangzhou 310027 P. R. China
  We reported a facilely prepared electrochemical composite biosensor for glucose featured with good sensing properties. The biosensor was constructed by simply depositing electropolymerized nanostructured polyaniline (PANI) onto a platinum needle and subsequent electrical decoration with an external layer of polydopamine (PDA) and glucose oxidase (GOx). The electrocatalytic behaviors and morphology of the composite biosensor were investigated. The PANI-PDA-GOx biosensor exhibited much higher sensitivity towards glucose with respect to PANI-GOx sensor with a detection limit of 3 μM and revealed fast response (response time ~ 10 s). Moreover, PANI-PDA-GOx showed much better long-term stability than PANI-GOx alone. It retained ~ 60% of the sensitivity to glucose after repeated testing and kept for about two months at -20°C. The improved sensing behavior of the composite biosensor is proposed to result from the introduction of PDA with good biocompatibility and crosslinking structure.
  Polyaniline; Dopamine; Glucose detection; Electrochemistry; Biosensor  

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