creating knowledge for future



High Quality


Scholarly Publishing
                CAREER NETWORK  
         
World's one of the largest Research
Career Network
Benefits
  •     Academic & Industry jobs
  •     Project funding
  •     Visiting faculty positions
  •     Visiting scientist positions
  •     Invited talks
  •     and more...  
   
Register FREE  
 
 
 
 
 
  Journal of Nanoscience Letters 2012, 2: 20
  Research Article
 
Acyclic anionic nucleolipids for DNA delivery
  Claire Céballosa, Salim Khiatib,c, Philippe Barthélémyb,c, Michel Camploa  
     
a Centre Interdisciplinaire des Nanomatériaux de Marseille CINaM, UPR-CNRS 3118, Université Aix-Marseille II, Luminy, case 913, 13288 Marseille cedex 09, France
b Université of Bordeaux, F-33076, Bordeaux, France
c INSERM U869, F-33076, Bordeaux, France

   
  Abstract  
  The synthesis of the acyclic nucleolipid 2-dimyristoyl-sn-glycerol thymine phosphate 5 (diC14-T) was achieved starting from thymidine 1 through conventional phosphoramidite chemistry. This simple synthetic strategy, which provides the pure (R) enantiomer is amenable to a large diversity of natural diacyl glycerol. Transfection experiments realized on mammalian cells in the presence of Ca2+ revealed that the acyclic anionic 1,2-dimyristoyl-sn-glycerol 5 (diC14-T) was as efficient as its cyclic analogue counterpart diC14-3’-dT to deliver plasmid DNA into cells.
     
  Keywords  
  Bioinspired material; Nucleolipids; Anionic transfection reagents; Gene delivery; Synthetic acyclic nucleoside; Lipids; Glycerol derivative  
     
   
   
   
   
     

  © 2016 Cognizure