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  Journal of Nanoscience Letters 2013, 3: 23
  Research Article
Sol-gel derived nanostructured zirconia for cholesterol detection
  Ajeet Kaushika,b, Pratima R. Solankia,c, Pandurang M. Chavhana, Shekhar Bhansalib, Bansi D. Malhotraa,d  
a Biomedical Instrumentation Section, Materials Physics & Engineering Division, National Physical Laboratory (CSIR), Dr. K. S. Krishnan Marg, New Delhi 110012, India
b Department of Electrical and Computer Engineering, Florida International University, 10555 West Flagler St. EC 3900, Miami, Florida FL 33174
c Amity Institute of Nanotechnology, Amity University Campus, Sector-125, Noida-201303, India
d Department of Biotechnology, Delhi Technological University, Shabad Daulatpur, Delhi-110047, India

  The sol-gel derived nanostructured zirconium oxide (nano-ZrO2) film has been fabricated onto indium-tin-oxide (ITO) coated glass to immobilize cholesterol oxidase (ChOx) to detect cholesterol. The oriented structural growth of nanoZrO2 (with respect to 100 planes) has been confirmed using low angle X-ray diffraction (XRD) studies. The results of optical and electrochemical studies suggest that three dimensional cationic nanoZrO2 platform provides an effective surface to ChOx resulting in enhanced electron transport to the bioelectrode. The low value of Michael-Mention (Km) constant suggests the good affinity of cholesterol to ChOx immobilized onto nanoZrO2/ITO electrode.
  Nanostructured ZrO2 ; Cholesterol oxidase ; Electrochemical biosensor  

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