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Journal of Nanoscience Letters 2013, 3: 31
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Research Article
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Paclitaxel-loaded PLA-PEG nanoparticles prepared by high-pressure homogenization for large industrial production
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Yongjun Liu, Donghua Liu, Peng Zhang, Peng Li, Lili Wang, Na Zhang
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School of Pharmaceutical Science, Shandong University, 44 Wenhua Xi Road, 250012 Ji’nan, People’s Republic of China
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Abstract |
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Nanoparticle (NP) formulations have been widely studied in recent years and showed great potential in the treatment of different diseases. However, only few NPs have been used in clinic because of the lacking of preparation method for large industrial production. The objective of this article was to find a method which could be used for large industrial production. High-pressure homogenization method which could provide a high throughput and continuous production was employed to prepare paclitaxel (PTX)-loaded PLA-PEG NPs. The prepared NPs had a spherical morphology, uniform particle size distribution (198.6 ± 24.6 nm), negative zeta potential (-8.66 ± 0.52 mv), high encapsulation efficiency (77.88 ± 1.20%) and drug loading (5.27 ± 0.14%) by the optimal prescription. PTX-loaded NPs showed a similar cytotoxicity to Hela cells at 48 h compared to Taxol® in vitro. Compared with Taxol®, the elimination half-life of PTX in PTX-loaded NPs group was remarkably prolonged from 5.16 to 22.756 h and the AUC was significantly increased from 3.364 to 5.883 mg/L*h which indicated that PTX-loaded NPs had longer therapeutic time compared to Taxol®. These results showed that the high-pressure homogenization was promising to be applied to prepare NPs for large industrial production.
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Keywords |
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Paclitaxel; High-pressure homogenization; Nanoparticles; PLA-PEG; Long-circulating
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