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Journal of Nanoscience Letters 2014, 4: 8
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Research Article
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Sol-gel derived SiO2-CeO2 nanocomposite films for triglyceride detection
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Akhilesh K. Guptaa, Ajeet Kaushikb,d, Pratima R. Solankic, Chetna Dhanda, Shekhar Bhansalib, B. D. Malhotrad
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a Biomedical Instrumentation Section, Materials Physics & Engineering Division, National Physical Laboratory (CSIR), Dr. K. S. Krishnan Marg, New Delhi 110012, India
b Department of Electrical and Computer Engineering, Florida International University, 10555 West Flagler St. EC 3900, Miami, Florida FL 33174, USA
c Amity Institute of Nanotechnology, Amity University Campus, Sector-125, Noida-201303, India
d Department of Biotechnology, Delhi Technological University, Shabad Daulatpur, Delhi-110047, India
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Abstract |
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Sol-gel derived silica (SiO2) film fabricated onto indium-tin-oxide (ITO) coated glass substrate has been used for self-assembly of cerium oxide (CeO2) nanoparticles to fabricate Nano CeO2-SiO2 nanocomposite. The NanoCeO2-SiO2/ITO electrode has been utilized to immobilize lipase (Lip) via physical adsorption for triglyceride detection. The response studies of Lip/CeO2-SiO2/ITO bioelectrodes carried out using CV reveal the linearity from 50 to 400 mg dl-1 with high sensitivity (2.28 mA mg-1 dl) and stability. A low value of apparent Michaelis-Menten constant (~ 2.4 mM) indicates high enzyme affinity to tributyrin.
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Keywords |
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Cerium oxide-Silicon oxide nanocomposites; Triglyceride biosensor
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