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Journal of Spectroscopy and Dynamics 2014, 4: 14
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Research Article
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Spectroscopic investigations on the molecular interactions between sodium dodecyl benzene sulfonate and trypsin
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Hao Chen, Lu Zhang, Hongxu Yang, Rutao Liu
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School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, Shandong Province, 27# Shanda South Road, Jinan 250100, P. R. China
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Abstract |
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We determined the effects of sodium dodecyl benzene sulfonate (SDBS) binding to trypsin by fluorescence spectroscopy, synchronous fluorescence spectroscopy and circular dichroism (CD). Based on the fluorescence spectra, the fluorescence intensity of trypsin was greatly quenched by SDBS due to the formation of a moderately strong complex. The enthalpy change (ΔH) and entropy change (ΔS) indicated that electrostatic interaction was the dominant intermolecular forces in the binding of SDBS to trypsin. Synchronous spectra also revealed the micro-environmental changes of the Tyr’s residues. As shown by the UV-vis absorption (UV) spectra and circular dichroism (CD) data, the skeleton structure of trypsin was made looser and its secondary structure was altered by the addition of SDBS. In addition, the activity of trypsin decreased along with the increase of SDBS, suggesting that SDBS had various effects on both the structure and function of trypsin, which could pose potential threats to organisms.
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Keywords |
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Trypsin; SDBS; Fluorescence; Toxicity evaluation
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