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  Sci. Lett. J. 2012, 1: 23  
  Research Article
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Variability of metallothionein forms in feral freshwater mussels  
  Halina I. Falfushynska, Lesya L. Gnatyshyna, Olga O. Turta, Oksana B. Stoliar  
Research Laboratory of Comparative Biochemistry and Molecular Biology, Ternopil National Pedagogical University, M. Kryvonosa Str. 2, Ternopil, 46027, Ukraine
  Metallothioneins (MTs) of bivalve molluscs are widely explored as biomarkers. However the reasons for the variability of MTs forms are studied scantly. The objective of this study was to examine MTs properties in feral freshwater bivalves Anodonta anatina from three sites (agricultural site (A), cooling pond of nuclear power plant (N) and forestry site (F)) during spring, summer and autumn. Additional to main, 8 kDa, MT peaks were shown by the size-exclusion chromatography on Sephadex G-50 at site N in spring and at sites F and A in summer. These forms were identified as one-domain form (α-domain, 4 kDa) and oligomeric form of β-domain (16 kDa). Ion-exchange chromatography showed the variability of MTs forms in the same groups. Cu-enriched MTs (group N) were more vulnerable than Zn, Cd-enriched MTs (groups A and F). The vulnerability of Anodonta MTs can be explored in the environmental biorisk assessment.  
  Bivalve mollusc; Metallothionein; Domain; Seasonality  
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