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  Sci. Lett. J. 2015, 4: 82  
  Research Article
  Full Text
13C NMR detection of metabolic mixtures enhanced by dynamic nuclear polarization  
  Maxime Yona,b, Julie Lalande-Martinb, Talia Harrisa, Illa Teab, Patrick Giraudeaub, Lucio Frydmana  
     
a Department of Chemical Physics, Weizmann Institute of Science, 76100 Rehovot, Israel
b Chimie et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), UMR 6230, Université de Nantes, BP 92208, 2 rue de la Houssinière, 44322 Nantes Cedex 03, France

   
  Abstract  
  Dynamic Nuclear Polarization (DNP) shows a high potential to boost the sensitivity of NMR experiments. Particularly promising is the dissolution DNP approach, which can increase the sensitivity of certain liquid-state NMR experiments by factors in excess of 104. Usual applications of this promising technique rely on polarizing a 13C-labeled tracer, and following the latter’s metabolic fate after injection into an MRI scanner. This paper explores a different aspect pertaining the use of hyperpolarized metabolites, with a preliminary report exploring the potential of dissolution DNP in metabolomics analyses. To this end synthetic samples involving several common metabolites were hyperpolarized, and the analytical performance of the ensuing DNP NMR experiment was evaluated under a variety of different experimental conditions. These analyses revealed average signal enhancements of ~5000x to 10000x for non-protonated 13C sites, with a repeatability better than 10% on the 13C NMR peak areas. These are promising results, opening interesting application perspectives in the field of metabolomics analyses of biological extracts.  
     
  Keywords  
  Nuclear magnetic resonance; 13C Spectroscopy; Dynamic nuclear polarization; Sensitivity enhancement; Metabolites; Quantitative metabolomics  
     
   
     
 
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