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Sci. Lett. J. 2015, 4: 206
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Research Article
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Full Text
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Sterilisation effects on electrospun fibres manufactured from different grades of poly(e-caprolactone)
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A. K. Ghaga,b, F. Tancred-Holmesa, A. Gibba, S. H. Cartmella, L. A. Boswortha
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a School of Materials, The University of Manchester, Oxford Road, Manchester, M13 9PL, UK
b School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK
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Abstract |
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Chemical grade poly(ε-caprolactone) (PCL) is commonly used in biomaterial and tissue engineering research and often sterilised using methods (alcohol immersion or ultraviolet radiation, for example) that may not fully eradicate microorganism contaminants, simply because they are more economical. Unfortunately, the regulatory bodies do not approve this grade of material or sterilisation processes for implantation into humans. As such, translation into a clinical setting could be considerably delayed. This study focused on the comparison of electrospun fibre scaffolds manufactured from PCL medical or chemical grades and subsequent sterilisation by gamma irradiation (25 kGy) or ethanol (50-100%v/v). Effects on physicochemical properties and biocompatibility in a short-term in vitro study were evaluated. Significant differences in fibre diameter and tensile properties were obtained; and cell number was greatest when cultured on gamma-irradiated medical grade PCL. This study highlights the importance of selecting appropriate materials and sterilisation processes early in the research project time-line.
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Keywords
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Sterilisation; Gamma irradiation; Ethanol; Electrospun fibre; Poly(ε-caprolactone) (PCL); Electrospinning
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